Review



mouse monoclonal primary anti serca antibody  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Santa Cruz Biotechnology mouse monoclonal primary anti serca antibody
    Mouse Monoclonal Primary Anti Serca Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 340 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monoclonal+serca2+antibody/SERCA2+Antibody/pm41369381-58-7-12
    Average 94 stars, based on 340 article reviews
    mouse monoclonal primary anti serca antibody - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Redox Regulation of SERCA2 Is Required for Vascular Endothelial Growth Factor-Induced Signaling and Endothelial Cell Migration
    Article Snippet: .. Cells were treated with VEGF for 5 min or • NO gas solution (10 μ M ) for 1 min. Lysates were incubated with a custom polyclonal SERCA2 antibody and immunoblotted with a monoclonal GSH antibody (Virogen) and a monoclonal SERCA2 antibody (Santa Cruz). .. HAECs plated on gelatin-coated glass coverslips were loaded with 2 μM Fura2-AM (Invitrogen) in the presence of 0.02% pluronic F127 (Invitrogen) in serum-free endothelial growth media, and right before the experiment were transferred to nominally Ca 2+ -free PSS supplemented with 2.5 mM probenecid (Alfa Aesar, Ward Hill, MA).



    Similar Products

    monoclonal serca2-atpase antibody  (Thermo Fisher)
    90
    Thermo Fisher monoclonal serca2-atpase antibody
    Monoclonal Serca2 Atpase Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monoclonal+serca2+antibody/monoclonal+mouse+anti+serca2+antibody+ma3+919/pm39387444-54-45-51
    Average 90 stars, based on 1 article reviews
    monoclonal serca2-atpase antibody - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    mouse monoclonal primary anti serca antibody  (Santa Cruz Biotechnology)
    94
    Santa Cruz Biotechnology mouse monoclonal primary anti serca antibody
    Mouse Monoclonal Primary Anti Serca Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monoclonal+serca2+antibody/SERCA2+Antibody/pm41369381-58-7-12
    Average 94 stars, based on 1 article reviews
    mouse monoclonal primary anti serca antibody - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    monoclonal anti serca2a antibody  (ABclonal Biotechnology)
    94
    ABclonal Biotechnology monoclonal anti serca2a antibody
    FBS-induced RyR2 ablation underlies impaired Wnt responsiveness in AC16 cells. ( A and B ) Cytosol and SR calcium levels of AC16 cells preincubated with H 2 O or CHX (1 μg/ml) for 15 min before treatment with Wnt modulators for 6 h. Error bars indicate SD ( n = 6). ( C and E ) Western blot analysis of RyR2 and <t>SERCA2a</t> levels in AC16 cells in the medium with or without FBS treated with SFRP2 (10 μg/ml) or LiCl (20 mM) for 6 h. Error bars indicate SD ( n = 3). ( D and F ) Relative RyR2 and SERCA2a mRNA levels in AC16 cells with or without FBS treated with SFRP2 or LiCl for 6 h. Error bars indicate SD ( n = 6). ( G ) Relative RyR2 mRNA level for ‘RyR2-reconstituted’ cells compared to AC16 cells without FBS. ( H and I ) Cytosol and SR calcium levels of AC16 cells in FBS medium (labeled ‘Without RyR2’) and ‘RyR2-reconstituted’ cells in FBS medium (labeled ‘With RyR2’). ( J and K ) Cytosol and SR calcium levels of ‘RyR2-reconstituted’ cells in FBS medium and AC16 cells without FBS. Error bars indicate SD ( n = 6).
    Monoclonal Anti Serca2a Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monoclonal+serca2+antibody/SERCA2+Rabbit+mAb/pmc13093114-132-0-3
    Average 94 stars, based on 1 article reviews
    monoclonal anti serca2a antibody - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    monoclonal mouse igg antibody against serca2  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology monoclonal mouse igg antibody against serca2
    FBS-induced RyR2 ablation underlies impaired Wnt responsiveness in AC16 cells. ( A and B ) Cytosol and SR calcium levels of AC16 cells preincubated with H 2 O or CHX (1 μg/ml) for 15 min before treatment with Wnt modulators for 6 h. Error bars indicate SD ( n = 6). ( C and E ) Western blot analysis of RyR2 and <t>SERCA2a</t> levels in AC16 cells in the medium with or without FBS treated with SFRP2 (10 μg/ml) or LiCl (20 mM) for 6 h. Error bars indicate SD ( n = 3). ( D and F ) Relative RyR2 and SERCA2a mRNA levels in AC16 cells with or without FBS treated with SFRP2 or LiCl for 6 h. Error bars indicate SD ( n = 6). ( G ) Relative RyR2 mRNA level for ‘RyR2-reconstituted’ cells compared to AC16 cells without FBS. ( H and I ) Cytosol and SR calcium levels of AC16 cells in FBS medium (labeled ‘Without RyR2’) and ‘RyR2-reconstituted’ cells in FBS medium (labeled ‘With RyR2’). ( J and K ) Cytosol and SR calcium levels of ‘RyR2-reconstituted’ cells in FBS medium and AC16 cells without FBS. Error bars indicate SD ( n = 6).
    Monoclonal Mouse Igg Antibody Against Serca2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monoclonal+serca2+antibody/IgG+Antibody/pm32435418__ml0c00047_si_001-26-20-30
    Average 96 stars, based on 1 article reviews
    monoclonal mouse igg antibody against serca2 - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    mouse monoclonal anti serca2 f 1  (Santa Cruz Biotechnology)
    94
    Santa Cruz Biotechnology mouse monoclonal anti serca2 f 1
    FBS-induced RyR2 ablation underlies impaired Wnt responsiveness in AC16 cells. ( A and B ) Cytosol and SR calcium levels of AC16 cells preincubated with H 2 O or CHX (1 μg/ml) for 15 min before treatment with Wnt modulators for 6 h. Error bars indicate SD ( n = 6). ( C and E ) Western blot analysis of RyR2 and <t>SERCA2a</t> levels in AC16 cells in the medium with or without FBS treated with SFRP2 (10 μg/ml) or LiCl (20 mM) for 6 h. Error bars indicate SD ( n = 3). ( D and F ) Relative RyR2 and SERCA2a mRNA levels in AC16 cells with or without FBS treated with SFRP2 or LiCl for 6 h. Error bars indicate SD ( n = 6). ( G ) Relative RyR2 mRNA level for ‘RyR2-reconstituted’ cells compared to AC16 cells without FBS. ( H and I ) Cytosol and SR calcium levels of AC16 cells in FBS medium (labeled ‘Without RyR2’) and ‘RyR2-reconstituted’ cells in FBS medium (labeled ‘With RyR2’). ( J and K ) Cytosol and SR calcium levels of ‘RyR2-reconstituted’ cells in FBS medium and AC16 cells without FBS. Error bars indicate SD ( n = 6).
    Mouse Monoclonal Anti Serca2 F 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monoclonal+serca2+antibody/SERCA2+Antibody/pmc12178789-3-0-5
    Average 94 stars, based on 1 article reviews
    mouse monoclonal anti serca2 f 1 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    serca2 atpase monoclonal antibody  (Thermo Fisher)
    90
    Thermo Fisher serca2 atpase monoclonal antibody
    FBS-induced RyR2 ablation underlies impaired Wnt responsiveness in AC16 cells. ( A and B ) Cytosol and SR calcium levels of AC16 cells preincubated with H 2 O or CHX (1 μg/ml) for 15 min before treatment with Wnt modulators for 6 h. Error bars indicate SD ( n = 6). ( C and E ) Western blot analysis of RyR2 and <t>SERCA2a</t> levels in AC16 cells in the medium with or without FBS treated with SFRP2 (10 μg/ml) or LiCl (20 mM) for 6 h. Error bars indicate SD ( n = 3). ( D and F ) Relative RyR2 and SERCA2a mRNA levels in AC16 cells with or without FBS treated with SFRP2 or LiCl for 6 h. Error bars indicate SD ( n = 6). ( G ) Relative RyR2 mRNA level for ‘RyR2-reconstituted’ cells compared to AC16 cells without FBS. ( H and I ) Cytosol and SR calcium levels of AC16 cells in FBS medium (labeled ‘Without RyR2’) and ‘RyR2-reconstituted’ cells in FBS medium (labeled ‘With RyR2’). ( J and K ) Cytosol and SR calcium levels of ‘RyR2-reconstituted’ cells in FBS medium and AC16 cells without FBS. Error bars indicate SD ( n = 6).
    Serca2 Atpase Monoclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monoclonal+serca2+antibody/pmc11476361-75-33-40
    Average 90 stars, based on 1 article reviews
    serca2 atpase monoclonal antibody - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    27311 1 ap mouse monoclonal anti serca2  (Proteintech)
    93
    Proteintech 27311 1 ap mouse monoclonal anti serca2
    FBS-induced RyR2 ablation underlies impaired Wnt responsiveness in AC16 cells. ( A and B ) Cytosol and SR calcium levels of AC16 cells preincubated with H 2 O or CHX (1 μg/ml) for 15 min before treatment with Wnt modulators for 6 h. Error bars indicate SD ( n = 6). ( C and E ) Western blot analysis of RyR2 and <t>SERCA2a</t> levels in AC16 cells in the medium with or without FBS treated with SFRP2 (10 μg/ml) or LiCl (20 mM) for 6 h. Error bars indicate SD ( n = 3). ( D and F ) Relative RyR2 and SERCA2a mRNA levels in AC16 cells with or without FBS treated with SFRP2 or LiCl for 6 h. Error bars indicate SD ( n = 6). ( G ) Relative RyR2 mRNA level for ‘RyR2-reconstituted’ cells compared to AC16 cells without FBS. ( H and I ) Cytosol and SR calcium levels of AC16 cells in FBS medium (labeled ‘Without RyR2’) and ‘RyR2-reconstituted’ cells in FBS medium (labeled ‘With RyR2’). ( J and K ) Cytosol and SR calcium levels of ‘RyR2-reconstituted’ cells in FBS medium and AC16 cells without FBS. Error bars indicate SD ( n = 6).
    27311 1 Ap Mouse Monoclonal Anti Serca2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monoclonal+serca2+antibody/SERCA2%2CATP2A2+Antibody/pmc11271478__41467_2024_50502_MOESM1_ESM-51-42-41
    Average 93 stars, based on 1 article reviews
    27311 1 ap mouse monoclonal anti serca2 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    monoclonal antibodies against sarcoplasmic reticulum ca2þ atpase  (Santa Cruz Biotechnology)
    94
    Santa Cruz Biotechnology monoclonal antibodies against sarcoplasmic reticulum ca2þ atpase
    FBS-induced RyR2 ablation underlies impaired Wnt responsiveness in AC16 cells. ( A and B ) Cytosol and SR calcium levels of AC16 cells preincubated with H 2 O or CHX (1 μg/ml) for 15 min before treatment with Wnt modulators for 6 h. Error bars indicate SD ( n = 6). ( C and E ) Western blot analysis of RyR2 and <t>SERCA2a</t> levels in AC16 cells in the medium with or without FBS treated with SFRP2 (10 μg/ml) or LiCl (20 mM) for 6 h. Error bars indicate SD ( n = 3). ( D and F ) Relative RyR2 and SERCA2a mRNA levels in AC16 cells with or without FBS treated with SFRP2 or LiCl for 6 h. Error bars indicate SD ( n = 6). ( G ) Relative RyR2 mRNA level for ‘RyR2-reconstituted’ cells compared to AC16 cells without FBS. ( H and I ) Cytosol and SR calcium levels of AC16 cells in FBS medium (labeled ‘Without RyR2’) and ‘RyR2-reconstituted’ cells in FBS medium (labeled ‘With RyR2’). ( J and K ) Cytosol and SR calcium levels of ‘RyR2-reconstituted’ cells in FBS medium and AC16 cells without FBS. Error bars indicate SD ( n = 6).
    Monoclonal Antibodies Against Sarcoplasmic Reticulum Ca2þ Atpase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monoclonal+serca2+antibody/SERCA2+Antibody/pm38572539-114-9-36
    Average 94 stars, based on 1 article reviews
    monoclonal antibodies against sarcoplasmic reticulum ca2þ atpase - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    anti serca2 rabbit monoclonal antibody  (Cell Signaling Technology Inc)
    94
    Cell Signaling Technology Inc anti serca2 rabbit monoclonal antibody
    FBS-induced RyR2 ablation underlies impaired Wnt responsiveness in AC16 cells. ( A and B ) Cytosol and SR calcium levels of AC16 cells preincubated with H 2 O or CHX (1 μg/ml) for 15 min before treatment with Wnt modulators for 6 h. Error bars indicate SD ( n = 6). ( C and E ) Western blot analysis of RyR2 and <t>SERCA2a</t> levels in AC16 cells in the medium with or without FBS treated with SFRP2 (10 μg/ml) or LiCl (20 mM) for 6 h. Error bars indicate SD ( n = 3). ( D and F ) Relative RyR2 and SERCA2a mRNA levels in AC16 cells with or without FBS treated with SFRP2 or LiCl for 6 h. Error bars indicate SD ( n = 6). ( G ) Relative RyR2 mRNA level for ‘RyR2-reconstituted’ cells compared to AC16 cells without FBS. ( H and I ) Cytosol and SR calcium levels of AC16 cells in FBS medium (labeled ‘Without RyR2’) and ‘RyR2-reconstituted’ cells in FBS medium (labeled ‘With RyR2’). ( J and K ) Cytosol and SR calcium levels of ‘RyR2-reconstituted’ cells in FBS medium and AC16 cells without FBS. Error bars indicate SD ( n = 6).
    Anti Serca2 Rabbit Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monoclonal+serca2+antibody/ATP2A2%2FSERCA2+Rabbit+mAb/pmc10998858-298-11-20
    Average 94 stars, based on 1 article reviews
    anti serca2 rabbit monoclonal antibody - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    FBS-induced RyR2 ablation underlies impaired Wnt responsiveness in AC16 cells. ( A and B ) Cytosol and SR calcium levels of AC16 cells preincubated with H 2 O or CHX (1 μg/ml) for 15 min before treatment with Wnt modulators for 6 h. Error bars indicate SD ( n = 6). ( C and E ) Western blot analysis of RyR2 and SERCA2a levels in AC16 cells in the medium with or without FBS treated with SFRP2 (10 μg/ml) or LiCl (20 mM) for 6 h. Error bars indicate SD ( n = 3). ( D and F ) Relative RyR2 and SERCA2a mRNA levels in AC16 cells with or without FBS treated with SFRP2 or LiCl for 6 h. Error bars indicate SD ( n = 6). ( G ) Relative RyR2 mRNA level for ‘RyR2-reconstituted’ cells compared to AC16 cells without FBS. ( H and I ) Cytosol and SR calcium levels of AC16 cells in FBS medium (labeled ‘Without RyR2’) and ‘RyR2-reconstituted’ cells in FBS medium (labeled ‘With RyR2’). ( J and K ) Cytosol and SR calcium levels of ‘RyR2-reconstituted’ cells in FBS medium and AC16 cells without FBS. Error bars indicate SD ( n = 6).

    Journal: Journal of Molecular Cell Biology

    Article Title: Canonical Wnt signaling affects calcium homeostasis in serum-treated AC16 cells through MLN-mediated SERCA2a regulation

    doi: 10.1093/jmcb/mjaf050

    Figure Lengend Snippet: FBS-induced RyR2 ablation underlies impaired Wnt responsiveness in AC16 cells. ( A and B ) Cytosol and SR calcium levels of AC16 cells preincubated with H 2 O or CHX (1 μg/ml) for 15 min before treatment with Wnt modulators for 6 h. Error bars indicate SD ( n = 6). ( C and E ) Western blot analysis of RyR2 and SERCA2a levels in AC16 cells in the medium with or without FBS treated with SFRP2 (10 μg/ml) or LiCl (20 mM) for 6 h. Error bars indicate SD ( n = 3). ( D and F ) Relative RyR2 and SERCA2a mRNA levels in AC16 cells with or without FBS treated with SFRP2 or LiCl for 6 h. Error bars indicate SD ( n = 6). ( G ) Relative RyR2 mRNA level for ‘RyR2-reconstituted’ cells compared to AC16 cells without FBS. ( H and I ) Cytosol and SR calcium levels of AC16 cells in FBS medium (labeled ‘Without RyR2’) and ‘RyR2-reconstituted’ cells in FBS medium (labeled ‘With RyR2’). ( J and K ) Cytosol and SR calcium levels of ‘RyR2-reconstituted’ cells in FBS medium and AC16 cells without FBS. Error bars indicate SD ( n = 6).

    Article Snippet: Monoclonal anti-SERCA2a antibody (ABclonal, A11692), monoclonal anti-RyR2 (Abcam, ab224803), polyclonal anti-MLN (Thermofisher, MYREGLN-101AP), monoclonal anti-tubulin antibody (Abcam, ab7291), and monoclonal anti-GAPDH (Abcam, ab8245) were used.

    Techniques: Western Blot, Labeling

    Wnt inhibition upregulates MLN expression to suppress SERCA2a activity. ( A ) Relative SERCA2a activity in AC16 cells in FBS medium. ( B ) Gene expression of known SERCA2a modulators in AC16 cells with or without FBS treated with different Wnt modulators (LiCl: 20 mM; SFRP2: 10 μg/ml; XAV939: 10 μM; LF3: 1 μM) for 6 h. ( C ) Relative MLN mRNA level in AC16 cells with FBS treatment. ( D ) Western blot analysis of MLN in AC16 cells with FBS treatment. ( E and F ) Cytosol and SR calcium levels of MLN-knockdown cell lines in FBS medium. ( G and H ) Cytosol and SR calcium levels of MLN-kd2 AC16 cells in FBS medium and AC16 cells without FBS. Error bars indicate SD ( n = 6).

    Journal: Journal of Molecular Cell Biology

    Article Title: Canonical Wnt signaling affects calcium homeostasis in serum-treated AC16 cells through MLN-mediated SERCA2a regulation

    doi: 10.1093/jmcb/mjaf050

    Figure Lengend Snippet: Wnt inhibition upregulates MLN expression to suppress SERCA2a activity. ( A ) Relative SERCA2a activity in AC16 cells in FBS medium. ( B ) Gene expression of known SERCA2a modulators in AC16 cells with or without FBS treated with different Wnt modulators (LiCl: 20 mM; SFRP2: 10 μg/ml; XAV939: 10 μM; LF3: 1 μM) for 6 h. ( C ) Relative MLN mRNA level in AC16 cells with FBS treatment. ( D ) Western blot analysis of MLN in AC16 cells with FBS treatment. ( E and F ) Cytosol and SR calcium levels of MLN-knockdown cell lines in FBS medium. ( G and H ) Cytosol and SR calcium levels of MLN-kd2 AC16 cells in FBS medium and AC16 cells without FBS. Error bars indicate SD ( n = 6).

    Article Snippet: Monoclonal anti-SERCA2a antibody (ABclonal, A11692), monoclonal anti-RyR2 (Abcam, ab224803), polyclonal anti-MLN (Thermofisher, MYREGLN-101AP), monoclonal anti-tubulin antibody (Abcam, ab7291), and monoclonal anti-GAPDH (Abcam, ab8245) were used.

    Techniques: Inhibition, Expressing, Activity Assay, Gene Expression, Western Blot, Knockdown

    Combined MLN knockdown and RyR2 overexpression restores canonical Wnt-mediated calcium regulation in FBS-treated AC16 cells. ( A ) Relative SERCA2a activity in FBS-treated AC16 cells and MLN-kd2 cells under various treatments. Cells were treated with the SERCA2a activator SAA (1 μM), the Wnt inhibitor SFRP2 (10 μg/ml), or the SERCA2a inhibitor Tg (100 nM) for 6 h. MLN knockdown significantly increased basal SERCA2a activity and abolished SFRP2-induced suppression. ( B ) Relative SERCA2a activity in MLN-kd2 cells treated with canonical Wnt inhibitors (SFRP2: 10 μg/ml; XAV939: 10 μM; LF3: 1 μM) or the SERCA2a activator SAA (1 μM) for 6 h. ( C and D ) Cytosol and SR calcium levels in modified AC16 cells (‘New AC16 cells’) with combined MLN knockdown and RyR2 overexpression, compared to standard FBS-free AC16 cells and FBS-treated AC16 cells. Cells were treated with the Wnt activator LiCl (20 mM) or the Wnt inhibitor SFRP2 (10 μg/ml) for 6 h. Error bars indicate SD ( n = 6).

    Journal: Journal of Molecular Cell Biology

    Article Title: Canonical Wnt signaling affects calcium homeostasis in serum-treated AC16 cells through MLN-mediated SERCA2a regulation

    doi: 10.1093/jmcb/mjaf050

    Figure Lengend Snippet: Combined MLN knockdown and RyR2 overexpression restores canonical Wnt-mediated calcium regulation in FBS-treated AC16 cells. ( A ) Relative SERCA2a activity in FBS-treated AC16 cells and MLN-kd2 cells under various treatments. Cells were treated with the SERCA2a activator SAA (1 μM), the Wnt inhibitor SFRP2 (10 μg/ml), or the SERCA2a inhibitor Tg (100 nM) for 6 h. MLN knockdown significantly increased basal SERCA2a activity and abolished SFRP2-induced suppression. ( B ) Relative SERCA2a activity in MLN-kd2 cells treated with canonical Wnt inhibitors (SFRP2: 10 μg/ml; XAV939: 10 μM; LF3: 1 μM) or the SERCA2a activator SAA (1 μM) for 6 h. ( C and D ) Cytosol and SR calcium levels in modified AC16 cells (‘New AC16 cells’) with combined MLN knockdown and RyR2 overexpression, compared to standard FBS-free AC16 cells and FBS-treated AC16 cells. Cells were treated with the Wnt activator LiCl (20 mM) or the Wnt inhibitor SFRP2 (10 μg/ml) for 6 h. Error bars indicate SD ( n = 6).

    Article Snippet: Monoclonal anti-SERCA2a antibody (ABclonal, A11692), monoclonal anti-RyR2 (Abcam, ab224803), polyclonal anti-MLN (Thermofisher, MYREGLN-101AP), monoclonal anti-tubulin antibody (Abcam, ab7291), and monoclonal anti-GAPDH (Abcam, ab8245) were used.

    Techniques: Knockdown, Over Expression, Activity Assay, Modification